Thermo Fisher Scientific HSF1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

PA3-017 detects heat shock factor 1 (HSF-1) from human, mouse, and rat tissues and cells.
It has been successfully used in Western blot, immunoprecipitation, immunofluorescence, ELISA, and gel super shift assays.
By Western blot, this antibody detects an ~83 kDa protein representing HSF-1 from NIH-3T3 cell lysate.
The immunogen is recombinant human HSF-1 protein expressed in E. coli.

Target Information

All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins.
Regulation of heat shock gene transcription is mediated by the transcriptional activator heat shock factor (HSF), which binds to heat shock response elements (HSEs).
HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation, found upstream of all heat shock genes.
Although HSEs are conserved across species, HSF proteins from yeast, Drosophila, and humans differ in molecular weight and show limited immunological cross-reactivity.

Two HSFs have been identified in human cells—HSF1 and HSF2—with 38% sequence identity.
HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA-binding activity. Upon stress, HSF1 transitions to a nuclear-localized trimeric state that binds DNA.
Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.