Thermo Fisher Scientific SEC61B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Pig, Rat

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SEC61B,uniProtId:P60467-1,ncbiNodeId:9615,antigenRange:1-20,antigenLength:96,antigenImageFileName:PA3-015_SEC61B_P60467-1_Rabbit.svg,antigenImageFileNamePDP:PA3-015_SEC61B_P60467-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2239072

Product Specific Information

PA3-015 detects human, canine and porcine, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.

PA3-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~14 kDa protein representing sec61 beta from pig microsomes.

The PA3-015 immunogen is a synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta. This sequence is completely conserved between human, mouse, and canine.

Target Information

The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.