Thermo Fisher Scientific HSP104 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Yeast

Published species

Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to C-terminal residues D(894) D D N E D S M E I D D D L D(908) of S. cerevisiae HSP104. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP104,uniProtId:P31539-1,ncbiNodeId:-23,antigenRange:894-908,antigenLength:908,antigenImageFileName:PA3-016A_HSP104_P31539-1_Rabbit.svg,antigenImageFileNamePDP:PA3-016A_HSP104_P31539-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1017672

Product Specific Information

PA3-016A detects HSP104 from yeast samples. This antibody does not detect HSP100 from prokaryotes, nor certain plants and insects including Drosophila.

PA3-016A has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~104 kDa protein from yeast.

The PA3-016A immunogen is a synthetic peptide corresponding to the C-terminal residues D(894) D D N E D S M E I D D D L D(908) of S. cerevisiae HSP104.

Target Information

Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.