Thermo Fisher Scientific Actin, Skeletal Muscle Polyclonal Antibody
Rabbit polyclonal antibody targeting skeletal muscle actin (ACTA1). Validated for WB and IHC applications. Recognizes cytoplasmic actin in skeletal muscle tissues. Supplied as liquid, unconjugated form, purified by antigen affinity chromatography. For ...
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Guinea pig, Human, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from N-terminus of human alpha skeletal muscle isoforms of actin (ACTA1, UniProt ID: P68133-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Skeletal muscle
- Cellular location: Cytoplasmic
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved and play key roles in cell motility, structure, and integrity. Alpha, beta, and gamma actin isoforms have been identified:
- Alpha actins: major constituent of the contractile apparatus
- Beta and gamma actins: involved in regulation of cell motility
This actin is an alpha actin found in skeletal muscle.
Mutations in this gene cause:
- Nemaline myopathy type 3
- Congenital myopathy with excess of thin myofilaments
- Congenital myopathy with cores
- Congenital myopathy with fiber-type disproportion
These diseases lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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