Thermo Fisher Scientific c-Met Monoclonal Antibody (3D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 18 publications 18 publications

Immunohistochemistry (IHC)

5-10 µg/mL

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

0.1-1 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3D4

Immunogen

Synthetic peptide derived from cytoplasmic domain of human c-Met protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533289

Product Specific Information

This antibody is specific for the cytoplasmic domain of the c-Met protein. On western blotting, this antibody recognizes a band at approximately 145 kDa. During development reactivity was confirmed with human HT-29 breast carcinoma lysates and c-Met cytoplasmic domain recombinant protein by both western blot, and with formalin-fixed, paraffin-embedded breast carcinoma tissues by immunohistochemistry.

For immunohistochemistry with formalin-fixed, paraffin-embedded tissues, heat-induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining. Recommended antibody incubation period is overnight at 4°C.

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.