Thermo Fisher Scientific Phospho-Huntington (Ser421) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is specific for phosphorylated human Huntington protein at the pS421 residue.
BLAST analysis indicates 100% homology of the immunizing sequence with Huntington homologues from chimpanzee, pig, and chicken.
Cross-reactivity with mouse and rat homologues may occur (one amino acid difference).
Minimal reactivity is expected with the non-phosphorylated form of the protein.

Target Information

Huntingtin is a disease gene linked to Huntington’s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. The disease is caused by an expanded trinucleotide repeat in the huntingtin gene, resulting in a polyglutamine repeat in the protein.
HD is an autosomal dominant disorder with mid-life onset, featuring psychiatric disorders, dementia, and involuntary movements (chorea), leading to death within 10–20 years.

The huntingtin locus spans 180 kb and consists of 67 exons. The gene is widely expressed and required for normal development.
It is expressed as two alternatively polyadenylated forms (~13.7 kb and ~10.3 kb) with different abundance in fetal and adult tissues.
The genetic defect may alter mRNA or protein function rather than eliminating transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.