Thermo Fisher Scientific TGF beta 1 Polyclonal Antibody
Rabbit polyclonal antibody against human TGF beta 1. Western blot, ICC/IF, ELISA, IP에 사용 가능. 고순도 친화 크로마토그래피 정제. -20°C 보관, 반복 동결/해동 피할 것. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:10,000 – 1:20,000 |
| Immunoprecipitation (IP) | 1:100 – 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TGF beta 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.99 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Rabbit Anti-TGF beta 1 affinity purified antibody is directed against human TGFβ1 protein. A BLAST analysis suggests reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFβ1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.
Target Information
TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-β is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of αV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-β binding proteins.
Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor (TGF-LAP), and a specific binding protein (LTBP). TGF beta-1 regulates proliferation, differentiation, adhesion, and migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, found either in a latent form (TGFB1 homodimer, LAP homodimer, and latent TGFB1-binding protein) or an active form (TGF beta-1 homodimer). The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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