Thermo Fisher Scientific TGF beta 1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Rabbit Anti-TGF beta 1 affinity purified antibody is directed against human TGFβ1 protein. A BLAST analysis suggests reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFβ1 from non-mammalian sources as only a single amino acid residue change is found within the immunogen sequence from many other organisms.

Target Information

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-β is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of αV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-β binding proteins.

Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor (TGF-LAP), and a specific binding protein (LTBP). TGF beta-1 regulates proliferation, differentiation, adhesion, and migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, found either in a latent form (TGFB1 homodimer, LAP homodimer, and latent TGFB1-binding protein) or an active form (TGF beta-1 homodimer). The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.