Thermo Fisher Scientific Complement C5a Monoclonal Antibody (2942)
인간 C5a/C5a des-Arg를 인식하는 mouse monoclonal antibody. Western blot, ELISA, IHC(F), 기능 분석에 적합. Protein G 정제, PBS + 0.1% BSA 용액, 보존제 없음. 4°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| ELISA | 1:50 |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2942 |
| Immunogen | Neo-epitope on C5a |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1073837 |
Product Specific Information
- MA1-40161 detects C5a/C5a des-Arg from human samples.
- Validated for Western blot, ELISA, immunohistochemistry (frozen), and functional assay applications.
- Detects a 14 kDa band in Western blot.
- Immunogen: Neo-epitope on C5a.
- Positive control: Eye tissue from AMD patient.
- Negative control: Eye tissue from non-AMD patient.
- Endotoxin level: <24 EU/mg.
- Note: Under certain conditions, C5 can expose epitopes normally found only in cleaved activation products.
Target Information
The protein encoded by this gene is the fifth component of complement, essential for inflammatory and cytolytic processes. It consists of alpha and beta polypeptide chains linked by a disulfide bridge. Upon cleavage by a convertase, the alpha chain releases C5a, an anaphylatoxin with spasmogenic and chemotactic activity. The remaining C5b fragment forms complexes with C6 and other components to create the membrane attack complex (MAC).
Mutations in this gene can cause complement component 5 deficiency, leading to recurrent infections, and have been associated with liver fibrosis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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