Thermo Fisher Scientific SQSTM1 Monoclonal Antibody (GT1478)
SQSTM1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. Human 및 Mouse 반응성. 단백질 G 정제, PBS/glycerol buffer, 1 mg/mL 농도. 연구용으로 NF-κB 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:200 | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT1478 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SQSTM1 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735371 |
Product Specific Information
Positive Control:
HepG2, A549, H1299, HCT116, Huh-7 (untreated), Huh-7 (3 µM Thapsigargin treatment for 12 hr), Huh-7 (3 µM Thapsigargin treatment for 24 hr), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 24 hr), 2C4, MEF, MNT-1
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-κB) signaling pathway.
The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate NF-κB activation in response to upstream signals.
Alternatively spliced transcript variants encoding either identical or different isoforms have been identified. Mutations in this gene are associated with sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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