Thermo Fisher Scientific eIF3h Monoclonal Antibody (GT35512)
Thermo Fisher Scientific의 eIF3h Monoclonal Antibody (GT35512)는 인간 및 마우스 반응성을 가진 단일클론 항체로, WB, IHC, ICC/IF에 사용 가능. 단백질 A로 정제된 액상 형태이며, eIF3H 단백질 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT35512 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human eIF3H/EIF3S3 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735173 |
Product Specific Information
- Positive Control: A431
- Predicted Reactivity: Rat (99%), Xenopus laevis (86%), Chicken (95%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, essential for multiple steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S pre-initiation complex (43S PIC).
It promotes mRNA recruitment and scanning for AUG recognition, and is involved in disassembly and recycling of post-termination ribosomal complexes, preventing premature joining of ribosomal subunits.
The eIF-3 complex selectively targets mRNAs related to cell proliferation, differentiation, and apoptosis, regulating translation through RNA stem-loop binding.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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