Thermo Fisher Scientific LCK Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

LCK is a member of the Src-family tyrosine kinases, essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates ITAM motifs in the zeta subunits, creating binding sites for the SH2 domains of ZAP70, which is subsequently phosphorylated and activated by LCK. Activated ZAP70 phosphorylates adaptor LAT, forming signaling platforms for downstream signaling.
Most LCK is localized to the plasma membrane, with a fraction associated with the Golgi apparatus, potentially contributing to Raf activation under weak TCR stimulation.
LCK contains N-terminal myristylation and palmitylation sites, a PTK domain, and SH2/SH3 domains for protein-protein interactions. It also regulates apoptosis induced by various stimuli, excluding death receptor pathways.
Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternative splice variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.