Thermo Fisher Scientific NGAL Polyclonal Antibody
NGAL 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, IHC, Flow cytometry, ELISA 등에 사용 가능. 고순도 항원 친화 크로마토그래피 정제, 동결건조 형태로 제공되며 인체·마우스·랫트 반응성. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
| ELISA | 1–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21–N198) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746706 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, also known as lipocalin 2, siderocalin, or neutrophil lipocalin) is a 25 kDa glycosylated single-chain monomer belonging to the lipocalin family. These proteins bind and transport small lipophilic molecules.
NGAL is released by activated neutrophils, forms dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B).
Low-level expression of NGAL occurs in various epithelia and increases during inflammation or cancer. Expression in the kidney is dramatically elevated by ischemic or nephrotoxic injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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