Takara Baculovirus titration kits

Accurate titering of baculovirus is a vital step to determine the multiplicity of infection (MOI), a critical parameter to achieve optimal and consistent protein yield with baculovirus gene expression. Traditional methods used to determine baculovirus titer, such as plaque assays and end-point dilution assays, are laborious.

Takara Bio offers two titration systems that are simple, accurate, and less time-consuming. These titration systems are compatible with all commonly available (AcMNPV-based) baculovirus expression systems. We suggest using our baculovirus titration kits in combination with our BacPAK Baculovirus Expression System.

Accurate titering of baculovirus is a vital step to determine the multiplicity of infection (MOI), a critical parameter to achieve optimal and consistent protein yield with baculovirus gene expression. Traditional methods used to determine baculovirus titer, such as plaque assays and end-point dilution assays, are laborious.

Takara Bio offers two titration systems that are simple, accurate, and less time-consuming. These titration systems are compatible with all commonly available (AcMNPV-based) baculovirus expression systems. We suggest using our baculovirus titration kits in combination with our BacPAK Baculovirus Expression System.

BacPAK Baculovirus Rapid Titer Kit

This kit determines baculovirus titers within 48 hr using a standard immunological assay. The rapid titer immunoassay uses a primary monoclonal antibody raised against an AcMNPV envelope glycoprotein (gp64) to accurately identify virus-infected cells. A secondary HRP-conjugated antibody stains the infected cells so the number of infected foci can be counted by light microscopy. This kit is suitable for use with any virus stock with a titer of more than 104 pfu/ml.

BacPAK qPCR Titration Kit

This kit provides an extremely fast (only 4 hr) and simple method for titrating your viral stocks. The kit uses a quick DNA purification step before determining viral genome content using qPCR and green intercalating dye technologies. Viral DNA and BacPAK control DNA are serially diluted and subjected to qPCR. The DNA copy number of each viral sample is then determined by comparing its Ct value to a standard curve generated by plotting the Ct values of the diluted control samples against their respective copy numbers.

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Overview

• Saves time by shortening baculovirus expression experiments up to six days
• Eliminates troublesome plaque assays
• Compatible with any AcMNPV-based baculovirus expression systems
• Rapid determination of viral titers
• Harvest, titer, and infect in a single day
• Accurate titers produce the most consistent infection results

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Applications

• Determine baculovirus titers, in order to optimize recombinant protein expression

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.