
Takara Whole-genome amplification
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단일 세포 또는 극소량 DNA(6–50 pg)로부터 전체 게놈을 고효율로 증폭하는 Takara PicoPLEX WGA 시리즈. CNV, SNP, NGS 분석에 최적화되어 있으며 높은 재현성과 자동화 용이성을 제공. 생식건강 및 변이 검출 연구에 적합.
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Takara PicoPLEX Whole-Genome Amplification (WGA) Family
The PicoPLEX WGA family of products consists of three versions. All systems can amplify DNA directly from cell lysates (1–10 cells) or isolated gDNA (less than 6 pg to 50 pg).
They are suitable for array CGH, qPCR, and NGS applications.
For reproductive health applications, the original PicoPLEX WGA Kit (Cat. # R30050) is recommended.
PicoPLEX WGA Kit (Cat. # R30050)
- Used for measuring copy number variation (CNV) with high accuracy and reproducibility.
- Recommended for PGT-A and other reproductive health applications.
PicoPLEX Single Cell WGA Kit (Cat. # R300671, R300672, R300673)
- Updated version of PicoPLEX WGA with higher yields and longer amplicons.
- Ideal for applications requiring high DNA yield.
PicoPLEX Single Cell WGA Kit v3 (Cat. # R300718, R300722, R300723)
- Latest version featuring a high-fidelity amplification enzyme.
- Suitable for mutation detection (SNVs) and CNV analysis.
- Provides excellent performance and base-level resolution.
Overview
- Reproducible results from single cells (or 6–50 pg of DNA)
- Superior reproducibility of allele representation
- Easy to use and automate
- Clear results across all resolutions (microarray, PCR)
Applications
- CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target or amplicon enrichment for mutation detection
- SNP genotyping
Suitable Inputs
| Sample Type | Quantity Range |
|---|---|
| Mammalian cells | 1–10 cells |
| Bacterial cells | 1,000–10,000 cells |
| Isolated DNA | 6–50 pg (human DNA) |
| Sorted chromosomes | — |
| DNA form | Intact or fragmented, single- or double-stranded |
Additional Product Information
Refer to the product’s Certificate of Analysis for details on:
- Storage conditions
- Product components
- Technical specifications
See the Kit Components List under the Documents tab for component details.
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