Thermo Fisher Scientific Phospho-Smad1/5 (Ser463, Ser465) Polyclonal Antibody
Smad1/5 단백질의 Ser463/465 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 Protein A 정제, 안정적인 액상 형태로 -20°C 보관.
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Thermo Fisher Scientific Phospho-Smad1/5 (Ser463, Ser465) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Smad family of proteins function in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at its C-terminal SSMS motif. Phosphorylated Smad2 forms a complex with Smad4, which accumulates in the nucleus to regulate gene expression.
In mammals, eight Smad proteins have been identified and divided into three functional groups:
- Receptor-activated Smads (R-Smads): Smad1, Smad2, Smad3, Smad5, Smad8
- Common mediator Smads (Co-Smads): Smad4
- Inhibitory Smads (I-Smads): Smad6, Smad7
Smad1, Smad5, and Smad8 are phosphorylated in response to BMP signaling, enabling complex formation with Smad4 and nuclear translocation to regulate TGF-beta target genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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