Thermo Fisher Scientific Phospho-STAT1 (Ser727) Polyclonal Antibody
인간, 마우스, 랫트에서 인식되는 STAT1 Ser727 인산화 특이적 폴리클로날 항체. IHC, ICC, ELISA 등 다양한 응용 가능. Protein A로 정제된 고순도 제품으로 -20°C 보관. 연구용으로 세포 신호전달 및 STAT1 활성 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of Ser727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. It can be activated by interferon-alpha, interferon-gamma, EGF, PDGF, and IL6. STAT1 regulates genes involved in cell growth, apoptosis, immune responses, and lipid metabolism. It plays a key role in mediating cell viability in response to various stimuli and pathogen exposure.
The STAT1 gene is located on chromosome 2 and is activated through phosphorylation by JAK tyrosine kinases, enabling nuclear translocation and transcriptional regulation. Among the seven STAT types, STAT1, STAT3, STAT5a, and STAT5b have broad activation profiles. STAT1 responds to multiple ligands, including IFN family (IFN-Alpha, IFN-Beta, IFN-Gamma, IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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