Thermo Fisher Scientific TWIST1 Monoclonal Antibody (2F8)
TWIST1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, ICC/IF, ELISA에 적합합니다. 인간 시료 반응성이 있으며, PBS 버퍼에 보존제가 없는 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.3 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F8 |
| Immunogen | TWIST1 (NP_000465, 100–202 a.a) partial recombinant protein with GST tag. MW of GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
PQSYEELQTQ RVMANVRERQ RTQSLNEAFA ALRKIIPTLP SDKLSKIQTL KLAARYIDFL YQVLQSDELD SKMASCSYVA HERLSYAFSV WRMEGAWSMS ASH
Target Information
TWIST1 acts as a transcriptional regulator. It inhibits myogenesis by sequestering E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction.
TWIST1 also represses proinflammatory cytokines such as TNFA and IL1B.
It regulates cranial suture patterning and fusion, and gene expression differentially depending on dimer composition.
Mutations in the gene are implicated in Saethre-Chotzen syndrome, characterized by coronal synostosis, brachycephaly, low front hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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