Thermo Fisher Scientific MYH9 Monoclonal Antibody (GT566)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT566

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MYH9. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MYH9,uniProtId:P35579-1,ncbiNodeId:9606,antigenRange:1960,antigenLength:1960,antigenImageFileName:MA5-27766_MYH9_P35579-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27766_MYH9_P35579-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735283

Product Specific Information

Positive Control: HeLa, MYH9-transfected 293T

Predicted Reactivity: Mouse (100%), Rat (100%), Dog (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton that plays essential roles in various cellular processes. Structurally, the MYH9 gene spans over 106 kilobases and includes 41 exons that translate into a protein of 1,960 amino acids. This protein is a part of a hexameric complex, which includes two heavy chains, two regulatory light chains, and two essential light chains. The NMHC IIA protein interacts with actin filaments and is involved in cellular activities such as cell migration, adhesion, division, and maintenance of cell shape. Functionally, mutations in MYH9 can result in a spectrum of autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), which include conditions such as May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome. These disorders are primarily characterized by macrothrombocytopenia (abnormally large platelets) and may lead to other complications such as hearing loss, renal failure, and cataracts later in life. MYH9 is also crucial in hematopoiesis, where its proper function is necessary for the survival and maintenance of hematopoietic stem and progenitor cells (HSPCs). Loss of MYH9 function disrupts normal hematopoiesis, leading to severe blood cell deficiencies and bone marrow failure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.