Thermo Fisher Scientific HMGB1 Monoclonal Antibody (GT427)
Thermo Fisher Scientific의 HMGB1 Monoclonal Antibody (GT427)은 인간, 생쥐, 랫트에 반응하며 Western blot, IHC, ICC/IF에 사용 가능. 단일클론 마우스 IgG1 항체로, Protein G 정제 및 PBS 버퍼에 보존. HMGB1 단백질 연구 및 염증 반응 기전 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT427 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735219 |
Product Specific Information
- Positive Control: 293T, SK-N-SH, IMR32, SK-N-AS, Mouse brain, Rat brain
- Predicted Reactivity: Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. Recent studies indicate that damaged, necrotic cells release HMGB1 into the extracellular milieu, where it functions as a proinflammatory cytokine.
Mouse HMGB1 is expressed as a 215 amino acid single-chain polypeptide containing three domains:
- Two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9–79; and box B, aa 89–162)
- A negatively charged 30 aa C-terminal acidic tail region
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The cytokine activity resides in the B box, while the A box is associated with transcription factor helix-loop-helix domains.
HMGB1 acts as an inflammatory mediator promoting monocyte migration and cytokine secretion, and mediates T cell–dendritic cell interactions. It signals through high-affinity receptors RAGE, TLR2, and TLR4.
HMGB1 is highly conserved and ubiquitous in nuclei and cytoplasm of nearly all cell types, serving as a key mediator of inflammation during sterile and infection-associated responses.
Additionally, HMGB1 functions as a DNA nuclear binding protein and is an early trigger of sterile inflammation in trauma-hemorrhage models via activation of TLR4 and RAGE. Elevated HMGB1 levels are reported during tissue injury, infection, endotoxemia, sepsis, collagen-induced arthritis, and ischemia-reperfusion injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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