Thermo Fisher Scientific HMGN1 Monoclonal Antibody (SPM515)
인간 HMGN1 단백질을 인식하는 Mouse IgM 단일클론 항체로, 파라핀 조직 IHC에 최적화됨. Protein A/G로 정제된 액상 제품이며, PBS(pH 7.6) + 1% BSA, 0.1% sodium azide 포함. 열매개 항원 회수 권장, tonsil 조직을 양성 대조로 사용 가능.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM515 |
| Immunogen | Spleen cells of patient with hairy cell leukemia |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2118349 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil tissue.
Target Information
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription.
HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates.
These proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of higher-order chromatin structures, facilitating access to DNA sequences.
Clustering of architectural elements such as HMG proteins and linker histone subtypes into distinct domains may lead to structural and functional heterogeneity along the chromatin fiber.
HMG-14 and HMG-17 are constitutive components of mouse oocyte and embryonic chromatin, linking chromatin structure to embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
- MA1-37941_HMGN1_P05114-1_House_mouse.svg
- MA1-37941_HMGN1_P05114-1_House_mouse_PDP.jpeg
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