Thermo Fisher Scientific Bcl-xL Monoclonal Antibody (7D9)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

-

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7D9

Immunogen

Synthetic peptide corresponding to N-terminus of mouse BCL-XL protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_1070620

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin`s lymphoma.

Target Information

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.