Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 421, eBioscience
인간 및 마우스 c-Maf 단백질을 인식하는 sym0F1 단클론 항체로, Brilliant Violet 421 형광으로 표지되어 있습니다. 세포 내 염색 및 유세포분석(Flow Cytometry)에 적합하며, 405 nm 레이저 사용 시 최적의 발광(423 nm)을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
Tested Application
- Flow Cytometry (Flow): 5 µL (0.5 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sym0F1 |
| Immunogen | Full-length E. coli-produced c-Maf |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3093962 |
Additional Available Formats
- Alexa Fluor 488
- Brilliant Violet 480
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems.
In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays a key role in Th17 and Tfh differentiation by driving IL-21 expression and regulating IL-23R and CXCR5.
Applications Reported:
- Intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Pre-diluted and tested using intracellular staining of mouse splenocytes with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
- Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is optimized for cytometers with a violet (405 nm) laser. Ensure instrument compatibility before use.
Buffer Recommendations for Multi-Color Panels:
- Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific polymer interactions.
Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
c-Maf is the cellular counterpart of oncogenic v-maf, belonging to the basic region leucine zipper domain transcription factor family.
It regulates IL-4 expression in Th2 cells, crystalline genes in lens fibers, insulin in islets, and IL-10 in macrophages.
Chromosomal aberrations involving maf are associated with certain multiple myelomas, and c-Maf is expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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