Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Brilliant Violet 421, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• Brilliant Violet 480
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems.
In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays a key role in Th17 and Tfh differentiation by driving IL-21 expression and regulating IL-23R and CXCR5.

Applications Reported:

• Intracellular staining followed by flow cytometric analysis.

Applications Tested:

• Pre-diluted and tested using intracellular staining of mouse splenocytes with the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
• Recommended use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is optimized for cytometers with a violet (405 nm) laser. Ensure instrument compatibility before use.

Buffer Recommendations for Multi-Color Panels:

• Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific polymer interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

c-Maf is the cellular counterpart of oncogenic v-maf, belonging to the basic region leucine zipper domain transcription factor family.
It regulates IL-4 expression in Th2 cells, crystalline genes in lens fibers, insulin in islets, and IL-10 in macrophages.
Chromosomal aberrations involving maf are associated with certain multiple myelomas, and c-Maf is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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