Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.25 µg)/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 532, Alexa Fluor 700, APC, Biotin, Brilliant UV 395, Brilliant UV 615, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 786, eFluor 450, FITC, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Blue 725, NovaFluor Blue 760, NovaFluor Blue 800, NovaFluor Red 685, NovaFluor Red 710, NovaFluor UV 765, NovaFluor Violet 690, NovaFluor Violet 800, NovaFluor Yellow 610, NovaFluor Yellow 690, NovaFluor Yellow 730, NovaFluor Yellow 810, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702.

Product Specific Information

Description:
The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5, also known as Burkitt lymphoma receptor 1, BLR1). CD185 is a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma. It is expressed on B cells, CD4⁺ T cells (but not Th1 or Th2), and a subset of memory (CD45RO⁺) T cells. In tonsil tissue, CD185 is expressed on nearly all CD4⁺ cells with activation markers such as CD69 and ICOS and plays a role in B cell help and antibody production.

This receptor is critical for lymphocyte trafficking, particularly T cell migration into B cell follicles in response to CXCL13, making CD185 a marker of follicular helper T cells. The MU5UBEE antibody cross-reacts with Rhesus macaque.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.25 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is excited by a 405 nm laser. Ensure that the cytometer is equipped to detect this fluorochrome.

Staining Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled receptor for the chemokine BLC. It is specifically expressed in Burkitt’s lymphoma and lymphatic tissues such as lymph node follicles and spleen. CXCR5 plays an essential role in B cell migration and is expressed in human blood, salivary gland, and various animal tissues including lymph node, spleen, and tonsil.

G-protein Coupled Receptors (GPCRs) share a structural motif of seven membrane-spanning helices and exist in active and inactive forms. They are key pharmacological targets, with drugs such as Zyprexa and Claritin developed as GPCR-targeted therapeutics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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