Thermo Fisher Scientific SMAD1/SMAD5 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.5 µg/mL
• View 3 publications

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

The Smad family of proteins function in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 forms a complex with Smad4, which accumulates in the nucleus to regulate gene expression.

In mammals, eight Smad proteins have been identified, divided into three groups:

• Receptor-activated Smads (R-Smads): Smad1, Smad2, Smad3, Smad5, Smad8
• Common mediator Smads (Co-Smads): Smad4
• Inhibitory Smads (I-Smads): Smad6, Smad7

Smad1, Smad5, and Smad8 are phosphorylated in response to BMP signaling, while Smad2 and Smad3 respond to TGF-beta and activin. Phosphorylation enables R-Smad binding to Smad4, facilitating nuclear translocation and regulation of TGF-beta target genes. I-Smads inhibit this process by blocking receptor or Smad4 interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.