Thermo Fisher Scientific SMAD4 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

The synthetic peptide sequence is 97–113aa, RLWRWPDLHKNELKHVK.
Add 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Smad4 is encoded by the Smad4 gene located on chromosome 18 in humans. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic (MAD) homolog 4.
Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) domains involved in DNA binding and protein interactions. Binding of TGF-beta superfamily ligands (including TGF-beta and BMP) to their receptors phosphorylates Smad1, 2, 3, 5, and 8 (R-Smad), which then form heterotrimers with Smad4 (co-Smad) and translocate to the nucleus.
Inhibitory Smads (Smad6 and Smad7) interact with activated R-Smads to attenuate signaling. Smad4 functions as a tumor suppressor by transcriptionally regulating target genes such as Cyclin D1 (downregulation) and collagen (upregulation), which inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.