Thermo Fisher Scientific Nhe-1 Polyclonal Antibody
Nhe-1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC(P) 실험에 사용 가능. E.coli 유래 SLC9A1 항원을 면역원으로 제작. 고순도 항원 친화 크로마토그래피 정제. 동결건조 형태로 제공되며, 재구성 시 500 µg/mL 농도 유지.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human SLC9A1 recombinant protein (Position: H543–Q815) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide |
| Storage conditions | −20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2747148 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The Na⁺/H⁺ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by various signals including growth factors, mitogens, neurotransmitters, and tumor promoters.
Nhe-1 acts as an anchor for actin filaments to maintain the integrity of the cortical cytoskeleton through structural links with actin-binding proteins ezrin, radixin, and moesin (ERM proteins).
A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton independent of its ion exchange function. It may also play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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