Thermo Fisher Scientific Connexin 26 Monoclonal Antibody (CX-12H10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 19 publications 19 publications

Immunohistochemistry (IHC)

-

View 25 publications 25 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

-

View 25 publications 25 publications

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bat, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

CX-12H10

Immunogen

Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533036

Product Specific Information

13-8100 reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5 kDa. Reactivity of this antibody on western blots has been confirmed by using extracts from mouse liver, mouse brain, and rat brain and it appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions.

Target Information

Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variables (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.