Norgen FFPE DNA Purification Kit
FFPE 조직 샘플에서 고품질 DNA를 신속하게 추출 및 정제하는 키트입니다. 스핀 컬럼 방식으로 간편하고 빠른 처리 가능. 단편화된 DNA도 효율적으로 회수하며, qPCR·SNP·STR 분석 등 다양한 후속 실험에 적합. 고수율·고순도 DNA 확보.
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- Norgen
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Norgen FFPE DNA Purification Kit
SKU: 47400
For the rapid and efficient extraction and purification of DNA from FFPE samples.
Features and Benefits
- Fast and easy processing using rapid and convenient spin-columns
- Isolate high quality and high yield DNA
- DNA is free of inhibitors and ready for downstream use including SNP and STR genotyping
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of nucleic acids and proteins, and embedding can cause fragmentation over time.
Norgen’s FFPE DNA Purification Kit partially reverses formalin modifications, yielding high-quality DNA suitable for qPCR, mutation screening, microarray analysis, sequencing, and genotyping. The protocol can be completed in as little as 1 hour.
Supporting Data
Figure 1. High Yields of DNA
Comparison of Total DNA Yield Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA was isolated from 10 mg of FFPE kidney blocks and quantified using NanoVue spectrophotometer (GE Healthcare). Norgen's kit produced a much higher DNA yield than the competitor.
OCR Data:
| Sample | Yield (µg) |
|---|---|
| Competitor | 12.13 |
| Norgen | 29.62 |
Figure 2. High Quality DNA
Comparison of DNA Quality Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA quality was assessed using A260:A280 and A260:A230 ratios. Norgen showed a higher A260:A230 ratio, indicating superior purity.
OCR Data:
| 항목 | 설명 |
|---|---|
| 범례 | Competitor (짙은 파란색), Norgen (밝은 파란색) |
| Y축 레이블 | Ratio |
| X축 항목 | 260/280, 260/230 |
Figure 3. qPCR Performance
Comparison of qPCR Performance from FFPE DNA Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA was isolated from 10 mg FFPE kidney blocks, and qPCR was performed using 500 ng DNA in a 2 µL reaction. Norgen and competitor kits showed similar Ct values, confirming high-quality DNA suitable for amplification.
OCR Data:
| Sample | Ct Value (approx.) |
|---|---|
| Competitor | ~20 |
| Norgen | ~19.5 |
Figure 4. Isolate All Sizes of DNA
Comparison of DNA molecular weight range isolated by Norgen's FFPE DNA Kit and a Leading Competitor Kit.
Norgen’s kit captured more total DNA and covered a larger molecular weight range, recovering both high molecular weight genomic DNA and smaller fragments.
OCR Data:
| 구분 | 내용 |
|---|---|
| M | Marker |
| Norgen | Sample band |
| Competitor | Sample band |
Kit Specifications
| Specification | Description |
|---|---|
| Maximum Column Binding Capacity (gDNA) | 10 μg |
| Maximum Loading Volume Per Spin Column | 650 μL |
| Size of DNA Purified | All sizes > 80 bp |
| Maximum Amount of Starting Material | 5 sections < 20 µm thick paraffin slices or 25 mg of unsectioned block |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.
Proteinase K should be stored at -20°C upon arrival and after reconstitution.
This kit is stable for 1 year after shipment.
Kit Components
| Component | Cat. 47400 (50 preps) |
|---|---|
| Digestion Buffer A | 25 mL |
| Buffer RL | 30 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 15 mL |
| Proteinase K | 12 mg |
| RNase A | 1 tube |
| gDNA Purification Micro Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Documentation
FAQs
Spin Column
Why do I have poor DNA recovery?
Possible causes:
- Incomplete lysis of cells or tissue
- Column clogging due to excess starting material
- Alternative elution solution used
- Missing ethanol or buffer additions
- Low DNA content in source tissue
Why is the column clogged?
Possible causes:
- Insufficient solubilization
- Exceeding maximum starting material
- Unclarified lysate used
- Centrifuge temperature below 15°C
Why is the RNA degraded?
Possible causes:
- Old FFPE sample
- RNase contamination
- Slow processing
- Improper RNA storage
- Excessive incubation at 80°C
- High RNase content in starting material
Why is the RNA not performing well in downstream applications?
Possible causes:
- Insufficient washing
- Ethanol carryover
- Incomplete formalin crosslink reversal
Citations
| Title | Citation | Authors |
|---|---|---|
| A novel viral infection in a Captive Colony of pelagic red crabs (Pleuroncodes planipes) from California | Journal of Invertebrate Pathology, 2025 | Elise E.B. LaDouceur et al. |
| Effect of the 16S rRNA Gene Hypervariable Region on the Microbiome Taxonomic Profile and Diversity in the Endangered Fish Totoaba macdonaldi | Microorganisms, 2024 | Itzel S. Pérez-Bustamante et al. |
| Gradient inflammation in the pancreatic stump after pancreaticoduodenectomy: Two case reports and review of literature | World Journal of Clinical Cases, 2024 | Tie-Gong Wang et al. |
| Tracking the emergence of a novel genotype of Decapod hepanhamaparvovirus in shrimp using laser microdissection and next generation sequencing | PLOS ONE, 2024 | Roberto Cruz-Flores, Arun K. Dhar |
| Detection of a novel microsporidium with intranuclear localization in farmed Penaeus vannamei from Latin America | Journal of Invertebrate Pathology, 2023 | Arun K. Dhar et al. |
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