Norgen FFPE DNA Purification Kit

Norgen FFPE DNA Purification Kit

SKU: 47400
For the rapid and efficient extraction and purification of DNA from FFPE samples.

---

Features and Benefits

• Fast and easy processing using rapid and convenient spin-columns
• Isolate high quality and high yield DNA
• DNA is free of inhibitors and ready for downstream use including SNP and STR genotyping

This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of nucleic acids and proteins, and embedding can cause fragmentation over time.
Norgen’s FFPE DNA Purification Kit partially reverses formalin modifications, yielding high-quality DNA suitable for qPCR, mutation screening, microarray analysis, sequencing, and genotyping. The protocol can be completed in as little as 1 hour.

---

Supporting Data

Figure 1. High Yields of DNA

Comparison of Total DNA Yield Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA was isolated from 10 mg of FFPE kidney blocks and quantified using NanoVue spectrophotometer (GE Healthcare). Norgen's kit produced a much higher DNA yield than the competitor.

OCR Data:

Sample	Yield (µg)
Competitor	12.13
Norgen	29.62

---

Figure 2. High Quality DNA

Comparison of DNA Quality Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA quality was assessed using A260:A280 and A260:A230 ratios. Norgen showed a higher A260:A230 ratio, indicating superior purity.

OCR Data:

항목	설명
범례	Competitor (짙은 파란색), Norgen (밝은 파란색)
Y축 레이블	Ratio
X축 항목	260/280, 260/230

---

Figure 3. qPCR Performance

Comparison of qPCR Performance from FFPE DNA Isolated by Norgen's FFPE DNA Kit and a Leading Competitor FFPE DNA Purification Kit.
DNA was isolated from 10 mg FFPE kidney blocks, and qPCR was performed using 500 ng DNA in a 2 µL reaction. Norgen and competitor kits showed similar Ct values, confirming high-quality DNA suitable for amplification.

OCR Data:

Sample	Ct Value (approx.)
Competitor	~20
Norgen	~19.5

---

Figure 4. Isolate All Sizes of DNA

Comparison of DNA molecular weight range isolated by Norgen's FFPE DNA Kit and a Leading Competitor Kit.
Norgen’s kit captured more total DNA and covered a larger molecular weight range, recovering both high molecular weight genomic DNA and smaller fragments.

OCR Data:

구분	내용
M	Marker
Norgen	Sample band
Competitor	Sample band

---

Kit Specifications

Specification	Description
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Loading Volume Per Spin Column	650 μL
Size of DNA Purified	All sizes > 80 bp
Maximum Amount of Starting Material	5 sections < 20 µm thick paraffin slices or 25 mg of unsectioned block

---

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.
Proteinase K should be stored at -20°C upon arrival and after reconstitution.
This kit is stable for 1 year after shipment.

---

Kit Components

Component	Cat. 47400 (50 preps)
Digestion Buffer A	25 mL
Buffer RL	30 mL
Wash Solution A	38 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
RNase A	1 tube
gDNA Purification Micro Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

---

Documentation

• Protocol (50 preps)
• Safety Data Sheet (SDS)
• Product Flyer

---

FAQs

Spin Column

Why do I have poor DNA recovery?

Possible causes:

• Incomplete lysis of cells or tissue
• Column clogging due to excess starting material
• Alternative elution solution used
• Missing ethanol or buffer additions
• Low DNA content in source tissue

Why is the column clogged?

Possible causes:

• Insufficient solubilization
• Exceeding maximum starting material
• Unclarified lysate used
• Centrifuge temperature below 15°C

Why is the RNA degraded?

Possible causes:

• Old FFPE sample
• RNase contamination
• Slow processing
• Improper RNA storage
• Excessive incubation at 80°C
• High RNase content in starting material

Why is the RNA not performing well in downstream applications?

Possible causes:

• Insufficient washing
• Ethanol carryover
• Incomplete formalin crosslink reversal

---

Citations

Title	Citation	Authors
A novel viral infection in a Captive Colony of pelagic red crabs (Pleuroncodes planipes) from California	Journal of Invertebrate Pathology, 2025	Elise E.B. LaDouceur et al.
Effect of the 16S rRNA Gene Hypervariable Region on the Microbiome Taxonomic Profile and Diversity in the Endangered Fish Totoaba macdonaldi	Microorganisms, 2024	Itzel S. Pérez-Bustamante et al.
Gradient inflammation in the pancreatic stump after pancreaticoduodenectomy: Two case reports and review of literature	World Journal of Clinical Cases, 2024	Tie-Gong Wang et al.
Tracking the emergence of a novel genotype of Decapod hepanhamaparvovirus in shrimp using laser microdissection and next generation sequencing	PLOS ONE, 2024	Roberto Cruz-Flores, Arun K. Dhar
Detection of a novel microsporidium with intranuclear localization in farmed Penaeus vannamei from Latin America	Journal of Invertebrate Pathology, 2023	Arun K. Dhar et al.

---

Related Products

FFPE RNA/DNA Purification Plus Kit

---

제품 이미지