Thermo Fisher Scientific AMPD1 Polyclonal Antibody
Rabbit polyclonal antibody specific to AMPD1, reactive with human, mouse, and rat. Validated for IHC, ICC/IF, and ELISA. Supplied as liquid, 1 mg/mL, purified by Protein A. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from mouse AMP deaminase 1 (amino acids 101–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
AMP deaminase (AMPD) is an allosteric enzyme involved in the regulation of adenosine metabolism. It catalyzes a key reaction in purine nucleotide biosynthesis, converting AMP to IMP and releasing ammonia.
There are three functional isoforms of AMPD. AMPD1 (E.C. No 3.5.4.6.) is the skeletal muscle-specific isoform (M type), localized in type II muscle fibers, neuromuscular junctions, and capillaries.
AMPD1 acts as a sensor of the cell's energy requirements. Deficiency in AMPD1 leads to irregular muscle metabolism, reduced ATP degradation, altered phosphocreatine hydrolysis, and lactic acid accumulation. Mutations in AMPD1 are associated with neuromuscular disorders, exercise-induced skeletal muscle myopathies, and congestive heart failure due to coronary artery disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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