Merck Anti-REST Antibody, clone 2D9.1

Anti-REST Antibody, clone 2D9.1

clone 2D9.1, from mouse

RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor

Quality Level

100

생물학적 소스

mouse

항체 형태

purified immunoglobulin

antibody product type

primary antibodies

클론

2D9.1, monoclonal

species reactivity

human

포장

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

NP_001180437

UniProt 수납 번호

Q13127

배송 상태

ambient

RE1-silencing transcription factor (UniProt: Q13127; also known as REST, Neural-restrictive silencer factor, X2 box repressor) is encoded by the REST (also known as NRSF, XBR) gene (Gene ID: 5978) in human. REST is a transcriptional repressor that acts by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE). It is ubiquitously expressed and higher expression is found in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. The protein is also found in undifferentiated neuronal progenitor cells and it is believed that it acts as a master negative regular of neurogenesis. REST restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. REST also mediates repression by recruiting the BHC complex at RE1/NRSE sites, which acts by deacetylating and demethylating specific sites on histones and acting as a chromatin modifier. REST is shown to contain nine zinc finger domains, but a C-terminally truncated form is also produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and has weaker DNA binding capacity. Mutations in REST gene can lead to pediatric malignancy of the kidney which occurs due to uncontrolled multiplication of renal stem, stromal, and epithelial cells.