Merck Collagenase from Clostridium histolyticum

Collagenase from Clostridium histolyticum

high purity, purified by chromatography, Type VII, ≥4 FALGPA units/mg solid, lyophilized powder, ≥700 CDU/mg solid (CDU = collagen digestion units)

Clostridiopeptidase A

Quality Level

200

유형

Type VII

form

lyophilized powder

specific activity

≥4 FALGPA units/mg solid
≥700 CDU/mg solid (CDU = collagen digestion units)

분자량

68-130 kDa

정제법

chromatography

구성

Protein, ≥85% biuret

solubility

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

외래 활성

caseinase and clostripain ≤1 units/mg protein

저장 온도

−20°C

Collagenase has been used in the preparation of arterial tissue for the study of advanced glycosylation end products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.