Merck Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas

Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein

Quality Level

300

생물학적 소스

bovine pancreas

유형

Type IV

form

lyophilized powder

specific activity

≥2,000 Kunitz units/mg protein

분자량

~31 kDa

정제법

chromatography

구성

Protein, ≥80%

solubility

0.15 M NaCl: soluble 5.0 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

외래 활성

Chymotrypsin ≤0.5%
Protease ≤0.05%
RNase ≤0.02%

배송 상태

wet ice

저장 온도

−20°C

DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the processing of rat brain tissue. This study showed that axonal growth on astrocytes is not inhibited by oligodendrocytes. In another study, thawed fixed samples of E. coli were digested with DNAse I from Sigma along with other enzymes. The digestion was done before permeabilization and staining of the nucleic acids.