Merck Trypsin from bovine pancreas

Trypsin from bovine pancreas

≥2,500 USP units/mg solid, meets USP testing specifications

Quality Level

300

agency

USP/NF
meets USP testing specifications

form

solid

specific activity

≥2,500 USP units/mg solid

분자량

23.8 kDa

정제법

crystallization

solubility

H2O: soluble
saline: soluble

application(s)

diagnostic assay manufacturing

저장 온도

−20°C

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.