Merck Anti-α-Tubulin antibody, Mouse monoclonal

Anti-α-Tubulin antibody, Mouse monoclonal

clone B-5-1-2, purified from hybridoma cell culture

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

purified from hybridoma cell culture

antibody product type

primary antibodies

클론

B-5-1-2, monoclonal

form

buffered aqueous solution

분자량

antigen ~50 kDa

species reactivity

chicken, kangaroo rat, sea urchin, rat, Chlamydomonas, bovine, human, African green monkey, mouse

향상된 검증

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

농도

~2 mg/mL

technique(s)

immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of human foreskin fibroblast cell line (FS11)

동형

IgG1

UniProt 수납 번호

P68366

application(s)

research pathology

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

α -tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α -subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the hybridoma B-5-1-2 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin).
Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Both subunits have a molecular weight of approx. 50 kDa and share considerable homology. In addition to α- and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven. Most of these tubulins have distinct subcellular localization and an emerging diverse set of functions. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Microtubular systems contain at least three α-tubulin isoforms. Two isoforms are coded by two αt-ubulin genes, which are both transcribed and code for extremely similar proteins. The third isoform is generated by post-translational modification.