Merck Monoclonal Anti-Calbindin-D-28K antibody produced in mouse

Monoclonal Anti-Calbindin-D-28K antibody produced in mouse

clone CB-955, ascites fluid

Quality Level

200

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

antibody product type

primary antibodies

클론

CB-955, monoclonal

분자량

antigen 28 kDa

포함

15 mM sodium azide

species reactivity

rat, bovine, guinea pig, human, mouse, canine, sheep, rabbit, feline, goat, pig

향상된 검증

independent
Learn more about Antibody Enhanced Validation

농도

15-55 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using rat cerebellum sections
indirect ELISA: suitable
western blot: 1:3,000 using MDBK cell extract

동형

IgG1

UniProt 수납 번호

P05937

배송 상태

dry ice

저장 온도

−20°C

Gene Information

human ... CALB1(793)
mouse ... Calb1(12307)
rat ... Calb1(83839)

Calbindin-D-28K is a highly conserved 28 kDa calcium binding protein with broad tissue distribution.

Monoclonal Anti-Calbindin-D-28K (mouse IgG1 isotype) is derived from the CB-955 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2.

Calbindin-D-28K belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain polypeptide folds of the EF-hand type for the acceptance of Ca2+. Calbindin is an attractive neuroanatomical marker. Despite its broad tissue distribution, calbindin-D-28K exhibits a cell-type specific expression pattern. Thus, it has been immunocytochemically localized in selected cells in many CNS structures, where it is thought to act either as an intracellular calcium buffer, or in the intramembranous transport of calcium. It is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells. Monoclonal antibody reacting specifically with calbindin-D-28K is instrumental in the localization of calbindin in different tissues and cell populations.