Merck SIG10 Chemically Competent Cells

SIG10 Chemically Competent Cells

for protein expression and DNA plasmid production

Grade

for molecular biology

form

buffered aqueous solution

세포 형질전환

competent cell type: chemically competent
transformation efficiency: ≥1 × 109 cfu/μg

배송 상태

dry ice

저장 온도

−70°C

The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α™ DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 109 cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 108 cfu/μg.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA