Merck OverExpress C41(DE3) pLysS Chemically Competent Cells

OverExpress™ C41(DE3) pLysS Chemically Competent Cells

for the highest protein expression

Grade

for molecular biology

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OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals.
The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications.

The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins.
The strain C41(DE3) was derived from BL21(DE3) and has at least one mutation that prevents cell death associated with expression of many recombinant toxic proteins.

The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins compared to C41(DE3).

OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, a natural inhibitor of T7 RNA polymerase.
Cells containing pLysS produce a small amount of T7 lysozyme, which suppresses basal expression of T7 RNA polymerase prior to induction, thereby stabilizing recombinants encoding particularly toxic proteins.

Genotype

F– ompT hsdSB (rB– mB–) gal dcm (DE3) pLysS (CmR)