Merck 3dGRO Organoid Dissociation Reagent

3dGRO™ Organoid Dissociation Reagent

Chemically Defined Enzyme-Free Organoid Dissociation Solution

Organoid Passaging Reagent

제품 정보

제품 개요

Organoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization, and exhibit organ functionality.
Organoids address the limitations of existing 2D model systems by providing:

• Similar composition and architecture to primary tissue: Organoids harbor small populations of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into all major cell lineages, with similar frequency as in physiological conditions.
• Relevant models of in-vivo conditions: Organoids are more biologically relevant to model systems and allow manipulation of niche components and gene expression.
• Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and differentiation capabilities of stem cell populations.

제품 설명

The 3dGRO™ Organoid Dissociation Reagent is a proprietary, chemically defined, enzyme-free dissociation solution used for passaging multiple organoid cell types (e.g., mouse intestinal organoids, human colon and lung organoids).
Due to its chemically defined nature, the reagent produces consistent organoid passaging results.

It can be used in combination with:

• 3dGRO R-Spondin-1 Conditioned Media Supplement (SCM104) for isolating and passaging primary intestinal organoids from mice.
• 3dGRO Human iPSC Derived Colon Organoid Expansion Medium (SCM304) for expanding human iPSC-derived colon organoids (SCC300).