Merck 3dGRO Human iPSC Derived Colon Organoids

3dGRO™ Human iPSC Derived Colon Organoids

제품 개요

Human intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types.
Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system.
They are useful tools to model intestinal development and disease, including:

• Colon cancer
• Celiac disease
• Inflammatory bowel diseases
• Host–microbiome interactions

Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).

제품 특징

3dGRO™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using Simplicon RNA reprogramming technology (Cat. No. SCR550).
Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids.

The organoids express colon-specific markers including:

• Posterior hindgut marker CDX2
• Carbonic anhydrase II (CA-II)
• Carbonic anhydrase IV (CA-IV)
• Goblet cell markers Mucin-2 and Mucin-5B

They can be propagated long-term by passaging every 10–12 days using 3dGRO™ Human Colon Organoid Expansion Medium (Cat. No. SCM304).

제품 사양

제품 이미지

(Figure 1. Colon organoid differentiation process)