Thermo Fisher Scientific Mouse HMGB1 (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species

Mouse

Published species

Not Applicable

Expression System

E. coli

Amino acid sequence

Mouse HMGB1, amino acids Met1-Glu215 (Accession # NM_010439) with an N-terminal His-tag

Tag

His-tag

Molecular weight

27.6 kDa

Class

Recombinant

Type

Protein

Purity

>98% by SDS-PAGE

Endotoxin concentration

<0.01 ng/µg

Activity

ED50 = 3 - 25 µg/mL; as measured by the induction of TNF-alpha expression in RAW264.7 cells in the presence of 10 µg/mL Polymyxin B.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

20mM tris HCl, pH 8.0, with 0.2M NaCl, 1mM DTT

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: High-mobility group box-1 (HMGB1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4.

Applications Reported: Mouse HMGB1 Recombinant Protein Carrier-Free is biologically active.

Applications Tested: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 µg/mL in the presence of 10 µg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.

Source: E. coli expressed amino acids Met1-Glu215 of mouse HMGB-1 accession # NM_010439.

Bioactivity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 µg/mL in the presence of 10 µg/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.

Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE.

Molecular Weight: The protein contains an N-terminal His tag and has a predicted molecular mass of 27.6 kDa.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.