Thermo Fisher Scientific Mouse TSLP (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

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View 3 publications 3 publications

Product Specifications

Published species

Not Applicable

Expression System

Insect cells

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Description: Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. Originally identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development, TSLP is expressed in thymus, spleen, kidney, lung, and bone marrow. TSLP activity overlaps with that of IL-7, playing a key role in the development of B cells, as well as stimulating the growth of thymocytes and T cells. TSLP stimulates monocytes to produce T cell-attracting chemokines such as TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines, including IL-4, IL-5, IL-13, and TNF alpha. This hematopoietic cytokine binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (CD127) and the TSLP-specific chain (TSLPR). Binding induces activation of STAT3 and STAT5 via phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant mouse TSLP is a 15.0 kDa protein consisting of 121 amino acid residues.

Applications Reported: Recombinant mouse TSLP is biologically active.

Applications Tested: This recombinant mouse TSLP has been tested in a Nag8/7 cell proliferation assay with an observed ED50 of 0.15 ng/mL, corresponding to a specific activity of approximately 6x10e6 Units/mg.

Source: Insect cell expressed N-terminal His-Tagged amino acids Tyr20-Glu140 of mTSLP accession #NM_021367.

Bioactivity: The ED50 tested in a Nag8/7 cell proliferation assay was 0.15 ng/mL, corresponding to a specific activity of approximately 6 x 10e6 Units/mg.

Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >99% as determined by SDS-PAGE.

Molecular Weight: 15-22 kDa on SDS-PAGE due to glycosylation.

Storage and handling: For best recovery, quick-spin vial prior to opening. Use in a sterile environment.

Target Information

TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.