Thermo Fisher Scientific Mouse GAPD (GAPDH) Endogenous Control (FAM/MGB probe, non-primer limited)

Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (FAM™/MGB Probe, Non-Primer Limited)

The Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (FAM™/MGB Probe, Non-Primer Limited) is designed as an endogenous control for relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with 6FAM™ dye and MGB quencher, and the primers are not limited. Suitable for singleplex PCR reactions.

This control is compatible with:

• Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays
• Custom TaqMan™ Gene Expression Assays
• Custom TaqMan™ Primers and Probes

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate the need for assay design and validation, providing pre-designed probe and primer sets for accurate normalization of RNA or DNA input amounts in gene expression studies.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify the selection of appropriate reference genes for human, mouse, rat, and other eukaryotic samples, reducing trial-and-error in experimental setup.

Simple to Use

• QC-tested and formulated into a single 20X mix
• Fully compatible with universal two-step RT-PCR conditions
• Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG)
• Compatible instruments: Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, 7000, and 7700 Sequence Detection Systems

Flexible Offering

Each endogenous control uses 5′ nuclease chemistry. Available configurations include:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM final) + two unlabeled primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM final) + two unlabeled primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM final) + two unlabeled primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for variations in cDNA input. Ensure consistent expression across all samples. For multiplexing, the control gene should be more highly expressed than the target.

Multiplex vs. Singleplex PCR

• VIC™-labeled probes are primer-limited and suitable for multiplexing.
• FAM™-labeled probes are non-primer limited and intended for singleplex use.

For Research Use Only. Not for use in diagnostic procedures.

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