Thermo Fisher Scientific Rat GAPD (GAPDH) Endogenous Control (VIC/MGB probe, primer limited)

Applied Biosystems™ Rat GAPD (GAPDH) Endogenous Control (VIC™/MGB Probe, Primer Limited)

The Applied Biosystems™ Rat GAPD (GAPDH) Endogenous Control (VIC™/MGB Probe, Primer Limited) is intended as an endogenous control. It enables relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with VIC™ dye and MGB, and the primers are limited. Suitable for multiplex or singleplex PCR reactions.

This endogenous control is designed for use with:

• TaqMan™ Gene Expression Assays
• TaqMan™ Custom Gene Expression Assays
• Custom TaqMan™ Primers and Probes

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate months of assay design, formulation, and testing. These pre-designed probe and primer sets enable normalization of sample RNA or DNA amounts in a reaction.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls help avoid trial-and-error in selecting controls for common human, mouse, rat, and eukaryotic genes.

Simple to Use

All components are QC tested, formulated into a single 20X mix, and functionally validated. Compatible with universal conditions for two-step RT-PCR. Use with TaqMan™ Universal PCR Master Mix and your cDNA sample for sensitive, reproducible quantitative gene expression data on Applied Biosystems instruments (7900HT, 7300, 7500 Real-Time PCR Systems, 7000, and 7700 Sequence Detection Systems).

Flexible Offering

Each endogenous control uses proven 5′ nuclease chemistry. Available dye and quencher combinations:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM) + two unlabeled primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM) + two unlabeled primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM) + two unlabeled primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for differences in cDNA input. Ensure consistent expression across all samples. For multiplexing, the endogenous control gene should be more abundantly expressed than the target gene.

Multiplex vs. Singleplex PCR

TaqMan™ Endogenous Controls with VIC™ reporter dye are primer limited, allowing multiplexing with target assays. Controls with FAM™ reporter dye are not primer limited and are intended for singleplex reactions.

For Research Use Only. Not for use in diagnostic procedures.

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