Thermo Fisher Scientific Axiom Strawberry FanaSNP 50k Genotyping Array, 384-HT format

Applied Biosystems™ Axiom™ Strawberry FanaSNP 50k Genotyping Array (384-HT format)

The Axiom FanaSNP Strawberry 50K Genotyping Array offers genome-wide coverage of polymorphic SNPs across cultivated and wild octoploid strawberry species. It is a component of the Axiom Agrigenomics Genotyping solution, where target genomic DNA is processed on the GeneTitan Multi-Channel instrument. The automated workflow includes hybridization, staining, washing, imaging, and genotyping data output for downstream analysis.
Also available in mini-96 format (Cat. No. 551042).

This array was designed by the University of California, Davis Strawberry Breeding Program in collaboration with the Affymetrix Expert Design Program.

Array Content

• Comprehensive content: 850,000 subgenome-specific SNPs from diverse octoploid strawberry accessions worldwide. 446,644 QC-passing polymorphic SNP genotype clusters showing disomic segregation are publicly available.
• Production-scale array design: 49,483 SNPs validated for production use; includes 5,809 SNPs from the iStraw 35K array for compatibility with previous platforms.
• Multiple reference genomes: SNP positions aligned to Camarosa v1 and updated to UCD Royal Royce (FaRR1) reference genomes.
• Global validation: Variants discovered by WGS resequencing of 47 F. x ananassa, 24 F. chiloensis, and 22 F. virginiana individuals; validated in 384 diverse octoploid strawberry accessions.

Applications

• Molecular genetics research
  • QTL discovery and genome-wide association
  • Kinship and parentage analysis
• Genomic and molecular breeding
  • Genomic prediction and selection
  • Marker-assisted selection
• Cultivar development
  • Accelerate cultivar release and breeding efficiency
  • Identify key markers in existing germplasm
  • Select germplasm suited for specific growing regions
• Intellectual property protection
  • Clonal source and purity confirmation
  • Parent-offspring relationship validation

Required Products

• GeneChip Command Console Software
• GeneTitan Multi-Channel Instrument
• Axiom Analysis Suite Software
• Axiom Long Format Export Tool (AxLE)

References

1. Hardigan et al. 2020; https://doi.org/10.3389/fpls.2019.01789
2. Edger et al. 2019; https://doi.org/10.1038/s41588-019-0356-4
3. Hardigan et al. 2022; https://doi.org/10.1101/2021.11.03.467115; https://phytozome-next.jgi.doe.gov/info/FxananassaRoyalRoyce_v1_0; https://datadryad.org/stash/dataset/doi:10.25338/B8TP7G

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