Thermo Fisher Scientific Phospho-SOX2 (Thr118) Polyclonal Antibody
Phospho-SOX2 (Thr118) 특이적 폴리클로날 항체로 인간, 마우스, 랫트 반응성. Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체. 줄기세포 연구 및 SOX2 인산화 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Sox2 (Thr-118) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-118 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Thr-126) and Sox3 (Thr-144), but low homology to other Sox family members. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942997 |
Product Specific Information
The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-118) peptide before affinity purification using phospho-Sox2 (Thr-118) peptide.
This antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells.
This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate.
The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach.
The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins.
Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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