Thermo Fisher Scientific Phospho-SOX2 (Thr128) Polyclonal Antibody
Phospho-SOX2 (Thr128) 특이적 토끼 다클론 항체로, 인간·마우스·랫트 반응성을 가집니다. Western blot과 면역세포화학에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 줄기세포 연구 및 신경 발달 관련 단백질 분석에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Sox2 (Thr-128) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-128 in mouse Sox2. This site is identical in human Sox2 (Thr-126), and has some homology with the conserved site in mouse Sox1 (Ser-136) and Sox3 (Ser-154). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942999 |
Product Specific Information
This antibody was cross-adsorbed to unphosphorylated Sox2 (Thr-128) peptide before affinity purification using phospho-Sox2 (Thr-128) peptide.
It detects a 34 kDa protein on SDS-PAGE immunoblots of mouse F9 stem cells.
This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in embryonic development and cell fate determination.
The SOX2 gene product is essential for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach.
SOX2 lies within an intron of another gene, SOX2 overlapping transcript (SOX2OT).
SOX2 protein may act as a transcriptional activator through complex formation with other proteins.
Mutations in SOX2 are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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