Thermo Fisher Scientific MXI1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 76-295 of human MXI1 (NP_5691572) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MXI1,uniProtId:P50539-3,ncbiNodeId:9606,antigenRange:76-295,antigenLength:295,antigenImageFileName:PA5-93131_MXI1_P50539-3_Rabbit.svg,antigenImageFileNamePDP:PA5-93131_MXI1_P50539-3_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806635

Product Specific Information

Immunogen sequence: LLEAASYLEQ IEKENKKCEH GYASSFPSMP SPRLQHSKPP RRLSRAQKHS SGSSNTSTAN RSTHNELEKN RRAHLRLCLE RLKVLIPLGP DCTRHTTLGL LNKAKAHIKK LEEAERKSQH QLENLEREQR FLKWRLEQLQ GPQEMERIRM DSIGSTISSD RSDSEREEIE VDVESTEFSH GEVDNISTTS ISDIDDHSSL PSIGSDEGYS SASVKLSFTS; Positive Samples: mouse kidney, rat kidney; Cellular Location: Nucleus

Target Information

It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1, Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad-binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2). It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.