Thermo Fisher Scientific CFTR Monoclonal Antibody (CF3)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA1-935 detects cystic fibrosis transmembrane conductance factor (CFTR) in human and mouse tissues.
It has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures.
By Western blot, this antibody detects a single ~170 kDa protein representing CFTR in T84 whole cell extract.
Immunofluorescence staining of CFTR in mouse epithelial cells with MA1-935 results in cell surface staining, consistent with localization at the plasma membrane.
This antibody also detects one or more immunologically related proteins in mouse cell line Heb7a that does not contain CFTR mRNA.
MA1-935 can also be used to inhibit the epithelial uptake of S. typhi in some mouse cell lines.

The immunizing peptide corresponds to amino acid residues 103–117 found in the first extracellular loop of human and rabbit CFTR.
This sequence is highly conserved in mouse, sheep, cow, and Xenopus laevis.

Target Information

Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations in the gene coding for the cystic fibrosis transmembrane conductance factor (CFTR), a cAMP-regulated chloride channel.
Approximately 70% of CF cases involve deletion of phenylalanine at position 508 (ΔF508), leading to abnormal chloride transport.
This mutation often causes severe lung and liver disease.
Interestingly, Salmonella typhi uses CFTR to enter intestinal epithelial cells, and ΔF508 heterozygote and homozygote mice show 86% and 100% reductions in S. typhi intestinal uptake, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.