Thermo Fisher Scientific Galectin 3 Monoclonal Antibody (A3A12)
Galectin-3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 시료 반응. 정제된 액상 형태로 1 mg/mL 농도, PBS에 sodium azide 포함. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 8 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | View publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 6 µg/mL | View publications |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | View 1 publication |
| ELISA | Assay-dependent | View publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A3A12 |
| Immunogen | Full length recombinant human galectin-3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2136775 |
Product Specific Information
MA1-940 detects galectin-3 from human, mouse, and rat tissues.
It has been successfully used in Western blot, immunohistochemistry, immunofluorescence, immunocytochemistry, and ELISA procedures.
- In Western blot, detects an ~30 kDa protein representing galectin-3 from Jurkat cells transfected with human galectin-3.
- Immunohistochemical staining of galectin-3 in rat olfactory bulb yields nuclear and plasma membrane staining.
- Not suitable for mouse NIH-3T3 in Western blot analysis.
- Detects a non-specific band in HeLa cell lysates at ~21 kDa.
- Antigen is full-length recombinant human galectin-3; epitope mapped to the first 58 amino acids.
Target Information
Galectin-3 is a galactose-specific lectin that binds IgE.
It may mediate endothelial cell migration stimulation via CSPG4 and integrin interactions.
Together with DMBT1, it is required for terminal differentiation of columnar epithelial cells during early embryogenesis.
In the nucleus, it acts as a pre-mRNA splicing factor.
Involved in acute inflammatory responses including neutrophil activation, adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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