Thermo Fisher Scientific TGF beta-1 Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

2.0-4.0 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Neutralization (Neu)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

Insect cells-derived Recombinant Human TGF-beta1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929125

Product Specific Information

AA Sequence of recombinant protein: discontinued.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human TGF-beta1. Anti-Human TGF-beta1-specific antibody was purified from tissue culture supernatant by Protein G affinity chromatography.

Western Blot: To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TGF-beta1 is 0.25-0.50 ng/lane, under reducing and non-reducing conditions.

Target Information

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.